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. 2016 Jun 10;291(32):16530–16540. doi: 10.1074/jbc.M116.717157

FIGURE 4.

FIGURE 4.

SCIMP is phosphorylated in response to zymosan stimulation. A, SCIMP phosphorylation in SCIMP immunoprecipitates (IP) prepared from BMDCs stimulated for 5 min by MHCIIgp cross-linking. WB, Western blotting. B, SCIMP phosphorylation in SCIMP immunoprecipitates prepared from BMDCs stimulated for 5 min with 300 μg/ml zymosan. C, localization of SCIMP-YFP into zymosan-containing phagosomes in BMDCs retrovirally transduced with a SCIMP-YFP coding construct. Zymosan is visible because of its strong autofluorescence. Scale bars = 10 μm. D, sustained SCIMP phosphorylation in SCIMP immunoprecipitates prepared from BMDCs stimulated for the indicated times with 300 μg/ml zymosan. E, sustained SCIMP phosphorylation in SCIMP immunoprecipitates prepared from BMMFs stimulated for the indicated times with 300 μg/ml zymosan.