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. 2016 Jun 9;291(32):16541–16552. doi: 10.1074/jbc.M115.710574

FIGURE 1.

FIGURE 1.

Human NK cells express mRNA for the α7, α4, and β2 nAChR subunits. Expression of α7, α4, and β2 nAChR subunits mRNA was assessed in human NK cells by RT-PCR. A, C, and E, transcripts of α7, α4, and β2 nAChR subunits were detected by electrophoresis in agarose gel as bands of 75, 121, and 136 bp, respectively (arrows); M, DNA ladder (50 bp (A) and 100 bp (C and E)); 1, PCR negative control; 2, PCR positive control (A, human α7 cDNA; B, human α4 cDNA; C, human β2 cDNA); 3, human NK cells. B, D, and F, relative quantification (RQ) of mRNA expression of α7, α4, and β2 nAChR subunit mRNA in human NK cells incubated with and without IL-12, IL-18, and IL-15 for 48 h. qPCR was carried out as described under “Experimental Procedures.” The results are the mean ± S.E., and are cumulative of at least three independent experiments performed with NK cells from different donors (n = 3–5). *, p < 0.05; ***, p < 0.001; paired t test.