Skip to main content
. 2016 Jun 9;291(32):16541–16552. doi: 10.1074/jbc.M115.710574

FIGURE 5.

FIGURE 5.

Activation of α7 nAChR down-regulates NKG2D expression in human NK cells. Human NK cells were stimulated for 48 h with IL-12/IL-18/IL-15, and further cultured for 18 h with cytokines in the absence (−) or presence of PNU-282987 (P), α-BTX and PNU-282987 (α-BTX + P) or α-BTX alone (α-BTX). Thereafter, cell surface expression of NKG2D, NKp46, and DNAM-1 was assessed by FC. The upper panels show expression of NKG2D (A), NKp46 (B) and DNAM-1 (C) in NK cells in the absence or presence of α7 nAChR ligands. Bar graphs show results as mean ± S.E., and are cumulative of three independent experiments performed with NK cells from different donors (n = 5). **, p < 0.01; one-way ANOVA test with Bonferroni post hoc test. The lower panels show representative histograms. Gray histogram, IC mAb; black line, NK cells stimulated with cytokines; dashed line, NK cells stimulated with cytokines and cultured in the presence of PNU-282987; gray line, NK cells stimulated with cytokines and cultured in the presence of α-BTX + PNU-282987.