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. 2016 Jun 15;291(32):16597–16609. doi: 10.1074/jbc.M116.723064

FIGURE 8.

FIGURE 8.

Interaction between IL-1Rrp2 and IL-1RAcP is increased in the presence of the IL-36 cytokine. A, molecular model of the interaction between the IL-1RAcP ECD and the IL-1Rrp2 ECD. B, chemical basis for the interaction between the ECDs of IL-1RAcP and IL-1Rrp2. The two molecules are shown with the positively charged residues in blue and the negatively charged residues in red. The blue arrow shows the position of the IL-1Rrp2 domain 3 in the absence of interaction with IL-1RAcP. C, IL-1RAcP forms a complex with the IL-1Rrp2. Upper panel: results of the co-IP assay with full-length IL-1Rrp2 and IL-1RAcP. Lower panel: results of a co-IP assay with IL-1Rrp2 ECD335 and IL-1RAcP. Where present, IL-36γ was added to cells at 20 ng/ml. D, interaction of IL-1RAcP ECD367 with IL-1Rrp2 ECD335. The cells were co-transfected with IL-1RAcP ECD367 along with either the full-length IL-1Rrp2 ECD335, truncated ECD258 or ECD126–211. After treated with IL-36γ, the complex in the culture medium was immunoprecipitated with anti-FLAG antibody and the interaction was detected by anti-HA antibody.