FIGURE 3.

Hepatic LRP1 deficiency impairs lipophagy. Cell lysates prepared from hLrp1^+/+ (filled bars) and hLrp1^−/− (open bars) hepatocytes after incubation with or without 250 μm palmitate were subjected to Western blotting with antibodies against LC3B (A) to detect LC3-I and LC3-II and p62 (B). Expression levels were determined by densitometry and normalized to the α-tubulin levels as arbitrary units (A.U.). Representative images of the blots are shown at the top of A and B. The hepatocytes after incubation were also examined by confocal microscopy, and representative images with quantification of LC3 and p62 punctae are shown in C and D. The data are presented as mean ± S.E. (error bars) of three independent experiments, each performed in duplicate. *, significant difference from the hLrp1^+/+ group at p < 0.01.