FIGURE 4.

Hepatic LRP1 deficiency activates ATF6 cleavage. Cell lysates prepared from hLrp1^+/+ (filled bars) and hLrp1^−/− (open bars) hepatocytes after incubation with or without 250 μm palmitate were subjected to Western blotting with antibodies against CHOP (A), cleaved ATF6 (B), phosphorylated and total IRE1α (C), and phosphorylated and total PERK (D). Anti-GADPH blots for GADPH levels were used as loading controls. Representative images of the blots are shown at the top of C and D, and the data are presented as mean ± S.E. (error bars) of three independent experiments, each performed with three biological replicates in each group. *, significant difference from the hLrp1^+/+ group at p < 0.01.