FIGURE 3.

Effect of sdAb-Fcs on total LDLR levels in hepatocytes. Primary hepatocytes isolated from PCSK9-deficient mice (A) or immortalized human hepatocytes (IHH; B) were incubated for 24 h in the absence (−) or presence of 0.08 μm (5 μg/ml) WT PCSK9 and increasing concentrations of sdAb-Fc. Cell lysates were subjected to WB analysis of LDLR and β-actin levels after separation by SDS-PAGE on 8% Tris-glycine gels. LDLR signals were calibrated to β-actin ones and were normalized to the values obtained in the absence of sdAb-Fc. The blots shown are representative of at least five independent experiments, each run as one biological sample per condition. The averaged normalized LDLR values are indicated below each blot. *, p < 0.05 (Student's t test).