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. 2016 Jun 13;291(32):16720–16729. doi: 10.1074/jbc.M115.710715

FIGURE 3.

FIGURE 3.

An OmpF-SurA-BamA ternary complex was detected by dual photo-crosslinking. A, immunoblotting results of the in vivo photo-crosslinking products for the BamA-K135Bpa variant, using the anti-SurA antibody. Cells were either untreated (lanes 1 and 2) or treated with chloramphenicol (a protein synthesis inhibitor) for 20 (lanes 3 and 4) or 40 (lanes 5 and 6) min before the in vivo photo-crosslinking. B, immunoblotting analysis for the photo-crosslinked OmpF-SurA-BamA ternary complex in cells co-expressing the BamA-K135Bpa and OmpF-Y32Bpa variant proteins. The immunoblotting was performed using antibodies against BamA (left) or SurA (right). The wild-type OmpF (with no Bpa incorporation) was also examined as a negative control (lanes 1, 2, 5, and 6). The positions of the photo-crosslinked OmpF-SurA-BamA, OmpF-SurA, and SurA-BamA complexes are indicated to the right of the gel.