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. 2016 Jun 1;291(32):16740–16752. doi: 10.1074/jbc.M116.717827

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Norcraugsodine reduction to norbelladine by NR. Enzymatic activity of NR for reduction of norcraugsodine was monitored using the same assay conditions and LC-MS/MS setup as in specific activity assays. A, MRM (m/z 260.1/238.0) of NR assays. Traces from top to bottom are: norbelladine standard; complete assay with NR, tyramine, 3,4-dihydroxybenzaldehyde, and NADPH; assay without NADPH; assay without tyramine and 3,4-dihydroxybenzaldehyde; assay without enzyme; and assay with TALON resin purified E. coli empty vector protein. B, MRM (m/z 260.1/238.0) relative quantification of assays shown in panel A in triplicate. C, EPI MS/MS of norbelladine standard. D, EPI MS/MS of NR product.