FIGURE 6.

A comparison of the tyramine (CE (15), DP (60), m/z 138.1/121.0 and m/z 138.1/93.0), levels in daffodil organs (above ground leaf (agl), below ground leaf (bgl), leaf scale (ls), bulb scale (bs), bulb core (bc), root (r), flower stalk above ground (fsag), flower stalk below ground (fsbg), and flower (f)). These samples were collected during the months of January (well developed roots with well developed flower primordia), March (all plants form this month lack flower primordia due to sampling limitations), April (plants all flowering), and August (dormant bulbs). The instrument used for the experiment was a QTRAP 6500 with a Phenomenex Luna 5-μm C8(2) 250 × 4.60-mm LC column using the following LC method; A = 0.1% formic acid and B = acetonitrile, start with 10% B for 2 min followed by a linear increase to 40% B for 9.9 min, linear increase to 90% B for 0.1 min, hold for 3 min, linear decrease to 10% B for 0.1 min, and hold 7 min. The following were also monitored by MRM across these conditions but were not detected 3,4-dihydroxybenzaldehyde (CE (15), DP (60), m/z 139.0/111.0 and 139.0/93.0), norbelladine (CE (15), DP (50), m/z 260.1/138.0 and 260.1/121.0), noroxomaritidine (CE (25), DP (70), m/z 272.3/229.0 and 272.1/212.1), and oxomaritinamine (CE (20), DP (60), m/z 274.1/219.1 and 274.1/136.1). Alkaloid samples were prepared by utilizing liquid nitrogen, a mortar and pestle for grinding of frozen tissue, followed by 70% ethanol extraction, PTFE membrane filtration, extract drying, and extract resuspension in mobile phase.