TABLE 1.
Activity summary of NR
Substrate | Product monitored | Specific activity |
---|---|---|
pmol min−1 mg protein−1 | ||
Noroxomaritidinea | Oxomaritinamine | 8600 ± 1250 |
8,9-O-Didemethyloxomaritidine | 9-O-Demethyloxomaritinamine | −b |
3,4-Dihydroxybenzaldehyde and tyraminea | Norbelladine | 21.2 ± 1.2 |
Isovanillin and tyraminea | 4′-O-Methylnorbelladine | 5.33 ± 0.21 |
Vanillin and tyramine | 3′-O-Methylnorbelladine | −b |
Piperonal and tyramine | 4-(2-((1,3-Benzodioxol-5-ylmethyl)amino)ethyl)phenol | −b |
a Measurements are with 500 μm substrate and 1 mm NADPH.
b Product made but not quantified. Values shown are mean ± S.D. for n = 3.