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Heparin is not released from sCT-OH upon incubation. sCT-OH was incubated with fl-hep at pH 5.5 for 22–45 min (dark gray to light) or mixed immediately before injection (black). An sCT·hep binding complex (peak at 20 min) forms immediately and disappears as fibrillation proceeds. The larger complex at 40 min was not consistently detected.
