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. 2016 Jun 8;291(32):16849–16862. doi: 10.1074/jbc.M116.715466

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Heparin induces ThT-positive sCT fibrils and increases the elongation rate at pH 7.4. A, sCT fibrillation followed by ThT fluorescence. Shown are sCT-OH (blue), sCT-NH₂ (red), and sCTH17A-OH (green) without heparin at pH 5.5 (open symbols) and with 1.5:1 hep_disacc:sCT (closed symbols). B, fibrillation of sCT-NH₂ and sCT-NH₂·heparin (1:1.6 molar ratio) at pH 7.4 followed by CD at 206 nm. The two conditions with/without heparin are each shown as duplicates to show the lag time variability. a.u., arbitrary units; mdeg, millidegrees.