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The expression of constructed IL-35 was determined by measuring protein levels. A, IL-35/EBI3 and IL-35/p35, linked by 3xGGGGS, was inserted into the NheI/XhoI sites of pcDNA3.1(−) to generate pCMV-IL-35. B, Western blots were performed to measure IL-35 in 293 cells transfected with pCMV-IL-35 for 48 h. C, Jurkat cells were electroporated with vector or pCMV-IL-35 for 36 h, and IL-35 protein levels in the supernatants were measured using ELISA. Ab, antibody. *, p < 0.05.
