Figure 6.

Modeling the initiation reaction. (A) Placement of TP priming residue Ser232 (in lemon green) and penultimate template nucleotide at the Φ29 DNAP active site during initiation of Φ29-DNA replication (catalytic aspartates and Mg²⁺ ions are shown in red and gray, respectively). Reproduced from de Vega and Salas (2011). (B) Schematic representation of the placement of the 3′ end of the template strand at the active site of Φ29 DNA polymerase. The templating nucleotide and the aromatic residue of the priming loop are indicated. This research was originally published in The Journal of Biological Chemistry. del Prado et al. (2015). Copyright©2015, by the American Society for Biochemistry and Molecular Biology.