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. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338

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Copyright © 2016, The Author(s)

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(a–c, left panels) Scheme of the exon structure of three genes targeted by CRISPaint. (a–c, right panels) Fluorescence imaging and deep sequencing analysis of ACTG1-mNeon (a) HIST1H4C-mNeon (b) and TUBB-mNeon (c) gene tagging using different frame selector plasmids. The frame selector predicted for in-frame tagging is marked in green. (d) Image quantification of mNeon tagging-positive cells. Shown are mean values+s.e.m. from three independent biological replicates. (e) Immunoblotting result of CRISPaint-mediated tagging of 16 human genes with a 3 × FLAG tag. Indicated below are the predicted protein sizes and frame selectors used.