Table 1.
Sequence of primer used in real time PCR analysis.
| Gene | Gene full name | GenBank accession No. | Forward primer | Reverse primer |
|---|---|---|---|---|
| S100a8 | S100 calcium binding protein A8 (calgranulin A) | NM_013650 | 5′TGCCCTCTACAAGAATGACT3′ | 5′AAGCTCTGCTACTCCTTGTG3′ |
| S100a9 | S100 calcium binding protein A9 (calgranulin B) | NM_009114 | 5′CGACACCTTCCATCAATACT3′ | 5′TCAGCATCATACACTCCTCA3′ |
| Il7 | Interleukin-7 | NM_008371 | 5′CGCAAGTTGAGGCAA TTTCT3′ | 5′ TTCCTTGCTTGTGCAGTTCA 3′ |
| Il2r | Interleukin-2 receptor | NM_008368 | 5′CTGGAGCCTGTCCCTCTACGTCTTCC 3′ | 5′GACCTGGGAGACCTTCCA GCTTATG3′ |
| Mmp9 | Matrix metallopeptidase 9 | NM_013599 | 5′TCCAGTACCAAGACAAAGCC 3′ | 5′TGA AGCAAA GAAGGAGCCC3′ |
| Mmp8 | connective tissue growth factor | NM_008611 | 5′GGTAACTAACTCTGCAGCCCTCTT3′ | 5′CGA ACCAGGGACGGA ATATG3′ |
| Wnt5a | Wingless-type MMTV integration site family, member 5A | NM_009524 | 5′TCCGGACTACTGTGTGC3′ | 5′AGCAGCACCAGTGAA AC3′ |
| Col1a2 | Collagen, type I, alpha 2 | NM_007743 | 5′TGAAGTGGGTCTTCCAGGTCTTTC3′ | 5′CACCCT TGTTACCGGATTCTCCTT3′ |
| Gsta2 | Glutathione S-transferase, alpha 2 | NM_008182 | 5′CCCCTTTCCCTCTGCTGAAG3′ | 5′TGCAGCCAC ACT AAA ACTTGA AAA3′ |
| Car3 | carbonic anhydrase 3 | NM_007606 | 5′GCTCTGCTAAGACCATCC3′ | 5′ATTGGCGAAGTCGGTAGG3′ |
| Glp2r | Glucagon-like peptide 2 receptor | NM_175681 | 5′TCATCTCCCTCTTCTTGGCTCTTAC3′ | 5′TCTGACAGATATGACATCCATCCA C3′ |
| Fabp6 | Fatty acid binding protein | NM_008375 | 5′ACGTGATTGAAAGGGGACGTAACTT3′ | 5′CATTCTTTGCCA ATGGTGAACTTGT3′ |
| Gys2 | Glycogen synthase 2 | NM_145572 | 5′CCAGCTTGACAAGTTCGACA3′ | 5′ATCAGGCTTCCTCTTCAGCA3′ |
| Igfbp5 | Insulin-like growth factor binding protein 5 | NM_010518 | 5′GTTACCCCGCCTCTCTTCC3′ | 5′TGTCTGAACGTA ACACTATAGAGAGC3′ |
| Ptpn1 | Protein tyrosine phosphatase, non-receptor type 1 | NM_011201 | 5′CGGCTATTTACCAGGACATTC3′ | 5′TGCGGTTGAGCATGACCAC3′ |
| E2f2 | E2F transcription factor 2 | NM_177733 | 5′ACGGCGCAACCTACAAAGAG3′ | 5′GTCTGCGTGTAAAGCGAAGT3′ |
| Sgsm1 | Small G protein signaling modulator 1 | NM_172718 | 5′ATGGAGGTGTCCAGCCTGAGAT3′ | 5′TGGTTTGTGCGTAGCAGGCATG3′ |
| Ube2c | Ubiquitin-conjugating enzyme E2C | NM_026785 | 5′GGTGACAAAGGAATCTCCGCCT3′ | 5′GGGAGAGTTTGTACCTCA GGTC3′ |
| β-tubulin | Tubulin, beta | NM_011655 | 5′CCGGACAGTGTGGCAACCAGATCGG3′ | 5′TGGCCAAAAGGACCTGAGCGAACGG3′ |