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. 2016 Aug 5;6:31011. doi: 10.1038/srep31011

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Copyright © 2016, The Author(s)

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(A) R28 cells were treated with HYPOX-4 (100 μM) and variable oxygen concentrations. HYPOX-4 dependent fluorescence increased with decreasing oxygen concentration. (B) R28, (C) ARPE19 and (D) MIO-M1 cells were treated with concentrations of HYPOX-4 ranging from 10 to 100 μM and 0.1% oxygen concentration; a HYPOX-4 dose-dependent fluorescence was observed in all of these cell types. (E,F) R28 cells were treated with HYPOX-4 (100 μM) and 0.1% oxygen for 4 hours. Hypoxia-specific fluorescence cell imaging was achieved. (G,H) Minimal fluorescence was observed in normoxic cells (n = 8, *p < 0.05).