Table 1. Fitting parameters of Ykt6 at different Ykt6:DPC-ratios.
Ykt6:DPC | Correlation | N | f | τD | p | τT | rel. N | ΔτD |
---|---|---|---|---|---|---|---|---|
1:0 | AC-d | 7.287 | 0.282 | 208.477 | 6.900 | 3.813 | 1 | 0 |
AC-a | 45.001 | 0.331 | 191.845 | 6.900 | 8.827 | 6.176 | −16.632 | |
CC-d | 110.008 | 454.090 | 6.853 | 15.097 | 245.613 | |||
CC-a | 99.619 | 469.967 | 6.900 | 13.671 | 261.490 | |||
1:2 | AC-d | 8.066 | 0.284 | 209.990 | 6.900 | 3.224 | 1 | 0 |
AC-a | 34.240 | 0.343 | 175.049 | 6.850 | 5.247 | 4.245 | −34.941 | |
CC-d | 95.760 | 210.056 | 6.898 | 11.872 | 0.066 | |||
CC-a | 68.955 | 229.725 | 6.898 | 8.549 | 19.735 |
AC-d stands for autocorrelation donor; AC-a stands for autocorrelation acceptor; CC-d is the crosscorrelation donor; CC-a is the crosscorrelation acceptor. N is the average number of molecules in the focal region; f is the triplet value; τD is the average transit time through the focal area; p is the ratio of lateral to axial dimensions of the focal region; τT represents the triplet dynamics of the dyes; rel.N and Δ τD reflect relative molecule number and transit time.