Table 2. Global fitting parameters of FCCS data at different Ykt6:DPC ratios.

Fittingparameter	Ykt6:DPC = 1:0	Ykt6:DPC = 1:2
N	9.11 ± 0.53	8.87 ± 0.67
τD (μs)	220 ± 37	224 ± 7
τi (μs)	190 ± 55	15 ± 32
α	0.13 ± 0.04	0.04 ± 0.05
β	0.12 ± 0.05	0.07 ± 0.11
γ	1.01 × 10−4 ± 0.91 × 10−4	0.10 × 10−2 ± 0.88 × 10−2
δ	0.25 ± 0.06	0.15 ± 0.06

N is the average number of molecules in the focal region; τ_D is the average transit time through the focal area; τ_i represents the time constant for the dynamic among the correlations which is the intramolecular dynamic; parameters α and γ are scaling factors due to the correlation amplitudes; β and δ represent static contributions from FRET states. Compare the τ_i value between different ration of DPC, it shows that when the ratio increases to 1:2, the value of τ_i is in the range of the error bound of apo system.