Table 1.
Virus | ORF | Predicted Function | Mutant Phenotype | Reporter Marker | Reference |
---|---|---|---|---|---|
FV3 | |||||
26R | eIF2α homologue | antagonist of PKR; IFNs; increased apoptosis; reduced pathogenesis | EGFP-puromycin resistance | [44] | |
82R | ICP-18 | increased apoptosis; increased induction of type I IFN; reduced pathogenesis | EGFP-puromycin resistance | “ | |
52L | β-hydroxysteroid dehydrogenase homolog | tbd; reduced pathogenesis | EGFP-puromycin resistance | [52] | |
64R | caspase activation & recruitment domain-containing (CARD) protein | IFNs; increased apoptosis; reduced pathogenesis | EGFP-puromycin resistance | “ | |
ATV | |||||
57R | eIF2α homologue | antagonist of PKZ; reduced pathogenesis | neomycin resistance | [27] | |
11R | unknown | essential gene | GFP-neomycin resistance | [37] | |
25R | RNase III | degrades RNA | GFP-neomycin resistance | “ | |
40L | CARD-containing gene | tbd; see FV3 above | GFP-neomycin resistance | “ | |
53R | Unknown—essential | essential gene | GFP-neomycin resistance | “ | |
54R | unknown | tbd | GFP-neomycin resistance | “ | |
RGV | |||||
53R | viral envelope protein | green virus | EGFP | [56] | |
92R | thymidine kinase (TK) | non-essential | EGFP | “ | |
53R | viral envelope protein | required for viral production; reduced growth when not expressed | IPTG inducible; EGFP | [60] | |
2L | viral envelope protein | required for viral production; reduced growth when not expressed | IPTG inducible; EGFP | [61] | |
92R67R | TK and deoxyuridine triphosphatase (dUTPase, DUT) | EGFP/RFP | [57] | ||
ESV | |||||
114L | dihydrofolate reductase (DHFR) | non-essential | EGFP-neomycin resistance | [58] | |
STIV | |||||
VP55 | viral envelope protein | green virus | EGFP-VP55 fusion | [43] |
tbd = to be determined; IFNs = interferon sensitivity; “ =same reference as above.