Figure 6.

Detection of duck Mx (duMx), duck OASL (duOASL), duck IFNα (duIFNα), duck IFNγ (duIFNγ), and duck IFNλ (duIFNλ) gene expression levels in DEFs. Cells were collected at 24 h and 48 h after the goose IFNα (goIFNα) and goose IFNγ (goIFNγ) protein pretreatment (60 µg/well). The mRNA levels were measured by real time PCR and are expressed relative to β-actin mRNA. Duck embryo fibroblast (DEF) cells were seeded in 6-well plates and then pretreated with by IFNα or IFNγ protein for 12 h. Symbols show four replicate experiments. The results shown are the mean ± SEM of four samples. Significant differences compared with cells without IFN treatment are denoted by * p < 0.05; ** p < 0.01 and *** p < 0.001.