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. 2016 Jul 20;8(7):199. doi: 10.3390/v8070199

Figure 2.

Figure 2

Screening the key domain of proteinase-polymerase (PP) required for the suppression of host gene expression. (A) Schematic diagram of the truncated PP constructs and the expression of each truncated mutant evaluated by Western blot using anti-Flag antibody; (B,C) CRFK cells were transfected with 0.25 μg of pFlag-PP and each truncated construct or empty vector together with 0.25 μg of the pIFN+33-Luc (B) or pHSV-TK-Luc (C) plasmids; At 24 h post-transfection, SeV (100 HA units) was inoculated into the pIFN+33-Luc transfection groups, and the luciferase assay was performed at 10 h post-inoculation. For the pHSV-TK-Luc transfection groups, the luciferase assay was performed directly at 24 h post-transfection. The positive controls were only transfected with 0.25 μg of pIFN+33-Luc (B) or pHSV-TK-Luc (C) Three independent experiments were performed and produced consistent results. The data represented the result of one experiment and were presented as means ± SD. ns, non-significant.