Figure 2.

Effect of virus concentration on inactivation by gamma irradiation. (A) Samples containing 10⁴, 10⁵, 10⁶, 10⁷, and 10⁸ TCID₅₀ units of rVSV-EBOVgp-GFP diluted with Dulbecco’s Modified Eagle Medium (DMEM) to a volume of 1 mL were subjected to 0.5 Mrad of gamma irradiation or left untreated (mock) on dry ice. Viral titers were determined by TCID₅₀ assay; (B) Plot of non-irradiated sample titers versus the reduction of titer following 0.5 Mrad (log₁₀((non-irradiated sample titer) – (irradiated sample titer)), means of data from (A)). Regression line equation shown. Data represent three independent experiments.