Figure 1.

mRNA expression of TNNT2 in the embryonic, neonatal and adult chick heart. PCR products obtained using a primer pair designed around the exon 5, which is alternately spliced in TNNT2. (A) Expression of a TNNT2 isoform in the embryonic chick heart (HH12–34; 235 bp). A smaller band of 178 bp can also be seen faintly expressed. GAPDH was used as a loading control (500 bp). (B) Two bands can be clearly seen in both the atrium and ventricle of the neonatal heart. In the adult atrium and ventricle, however, the lower band has a stronger signal than the upper 235 bp band. GAPDH was used as a loading control. + indicates RT; −, noRT; bp, base pairs; H₂O, PCR control.