Table 1.

Summary of phenotypic analysis after treatment with TNNT2 morpholino

Concentrationof morpholino	Embryo typea	External heart shape			Internal heart structures				Total internal
					Atrial septum		Trabeculation
		Diverticulum present	Normal	Total external	Reduced	Normal	Reduced	Normal
500 μm	Control	–	84	84	1	37	–	38	38
	TNNT2	2b	65	67	23	28	6	45	51
250 μm	Control	–	34	34	–	15	–	15	15
	TNNT2	–	43	43	–	19	–	19	19

Assessment of heart shape based on qualitative analysis of gross morphological features.

Qualitative assessment of atrial septa and degree of trabeculation obtained from serial histological sections.

TNNT2 denotes embryos treated with the TNNT2 ATG start site morpholinos.

^aControl embryos include standard and untreated controls.

^bBoth embryos with diverticula had reduced atrial septal size.