Figure 4.

The canonical Notch ligand genes Jag1 and Jag2 are expressed by chicken olfactory receptor neurons. Parasagittal sections of the embryonic chicken olfactory system. (A,B) At E6.0, Jag1 is expressed throughout the olfactory epithelium. (B¹–B⁴) Same section as (B) immunostained for Tubb3‐positive axons and Elavl3/4‐positive neuronal cell bodies, with a false‐colour overlay of Jag1, reveals that Jag1 is expressed by Elavl3/4‐positive olfactory receptor neurons (black/white arrowheads) and also non‐neuronal supporting cells (yellow arrowheads). (C–D⁴) At E6.5, Jag2 is expressed by Elavl3/4‐positive olfactory receptor neurons in the intermediate layer (black/white arrowheads), by a few Elavl3/4‐negative apical support cells (yellow arrowheads), and by migrating Elavl3/4‐positive neurons on the olfactory nerve (black/white arrows). Jag2 expression can also be seen in the vasculature, most likely in endothelial cells. (E–F¹) At E8.5, Jag2 expression is maintained in olfactory receptor neurons in the intermediate layer of the olfactory epithelium. Expression is also seen in the vasculature, most likely endothelial cells. Elav, Elavl3/4; FB, forebrain; OE, olfactory epithelium; ON, olfactory nerve. Scale bar: 100 μm.