Table 1. Bacterial strains and plasmids used in this study.
| Strain or plasmid | Relevant characteristics or purposea | Reference or source |
|---|---|---|
| P. aeruginosa | ||
| PA14 | wild type laboratory strain; RifR | Lab collection |
| katA | PA14 with in-frame deletion of katA; RifR | Lee et al.6 |
| oxyR | PA14 with in-frame deletion of oxyR; RifR | Choi et al.32 |
| anr | PA14 with in-frame deletion of anr; RifR | This study |
| rpoS | PA14 with in-frame deletion of rpoS; RifR | Park et al.33 |
| anrrpoS | anr with in-frame deletion of rpoS; RifR | This study |
| narG | PA14 with MAR2 × T7 insertion at narG; RifR, GmR | Liberati et al.34 |
| nirS | PA14 with MAR2 × T7 insertion at nirS; RifR, GmR | Liberati et al.34 |
| norB | PA14 with MAR2 × T7 insertion at norB; RifR, GmR | Liberati et al.34 |
| nosZ | PA14 with MAR2 × T7 insertion at nosZ; RifR, GmR | Liberati et al.34 |
| dnr | PA14 with MAR2 × T7 insertion at dnr; RifR, GmR | Liberati et al.34 |
| p1m | PA14 with the chromosomal mutation (CATCCT to GGTACC)b at the −10 box of katAp1; RifR | This study |
| p2m | PA14 with the chromosomal mutation (CACGCT to GGATCC)b at the −10 box of katAp2; RifR | This study |
| p1p2m | p1m with the chromosomal mutation (CACGCT to GGATCC)b at the −10 box of katAp2; RifR | This study |
| E. coli | ||
| DH5α | multi-purpose cloning | Lab collection |
| S17-1 | conjugal transfer of mobilizable plasmid; TpR; SmR | Lab collection |
| Plasmids | ||
| pEX18T | Positive selection suicide vector for allelic exchange; CbR | Lab collection |
| pEX18T-∆anr | pEX18T with the in-frame deletion in the anr gene; CbR | This study |
| pEX18T-katAp1m | pUCP18 with the −10 box mutation (CATCCT to GGTACC)b of katAp1; CbR | This study |
| pEX18T-katAp2m | pUCP18 with the −10 box mutation (CACGCT to GGATCC)b of katAp2; CbR | This study |
| pQF50 | lacZ transcriptional fusion; CbR | Farinha and Kropinski35 |
| pQF50-katAp | pQF50 with the katA promoter; CbR | This study |
| pQF50-katAp1m | pQF50 with the −10 box mutation (CATCCT to GGTACC)b of katAp1; CbR | This study |
| pQF50-katAp2m | pQF50 with the −10 box mutation (CACGCT to GGATCC)b of katAp2; CbR | This study |
| pQF50-katAp1p2m | pQF50 with the −10 box mutation (CATCCT to GGTACC)b of katAp1 and (CACGCT to GGATCC)b of katAp2; CbR | This study |
aRif R, rifampicin-resistant; GmR, gentamicin-resistant; CbR, carbenicillin- and ampicillin-resistant; TpR, trimethoprim-resistant; SmR, stremptomycin-resistant.
bunderlines; mutated nucleotides at the presumable −10 boxes.