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. 2016 Aug 5;36(4):e00362. doi: 10.1042/BSR20160141

Figure 2. High glucose promotes the cytoplasmic translocation of Dnmt3a in hMSCs.

Figure 2

(A) Immunofluorescence staining of Dnmt3a (green) under low glucose, mannitol or high glucose conditions for 24 h were shown. DAPI (blue) was used to stain the nucleus. At least 10 microscopic fields were assessed in each experiment. Images are representative of three independent experiments. Scale bar, 10 μm. (B) Western blot analysis of cytoplasmic and nuclear Dnmt3a protein levels at 24 h post high glucose treatment. β-Actin was used as the internal control for cytoplasmic portion and Lamin B was used as the internal control of nuclear portion. n=3 and representative image was shown. (C) Western blot analysis of total Dnmt3a protein levels at 0, 12, 24, 48 or 72 h post high glucose treatment. n=3 and representative image was shown.