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. 2016 Aug 5;36(4):e00362. doi: 10.1042/BSR20160141

Figure 3. High glucose decreases the binding of Dnmt3a to the CpG island of CTGF promoter.

Figure 3

(A) ChIP assay with anti-Dnmt3a antibody performed 24 hours after high glucose, mannitol, or low glucose treatment. n=3 and representative image was shown. (B) Quantification of ChIP assay based on three independent experiments. **P<0.01. (C) Methylation specific primer was use to amplify methylated CpG island of CTGF promoter at 12 and 24 h after high glucose, low glucose or mannitol treatment. n=3 and representative image was shown. (D) Quantification of methylation of CpG island of CTGF promoter based on 3 independent experiments. **P<0.01.