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. 2016 Aug 1;7:12341. doi: 10.1038/ncomms12341

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Copyright © 2016, The Author(s)

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(a) Detection of ¹³C-labelled asc-C7 and asc-C9 by LC–MS/MS in SRM mode in larvae injected or fed with ¹³C-labelled glucose (n=20). There were two peaks for labelled asc-C7: left arrow: asc-C7 with only sugar labelled (m/z 281.31), right arrow: asc-C7 with sugar labelled and side chain completely labelled (m/z 288.32). There were two peaks for labelled asc-C9: left arrow: asc-C9 with only sugar labelled (m/z 309.37), and right arrow: asc-C9 with sugar labelled and side chain completely labelled (m/z 318.37). The percentages of labelled asc-C7 and asc-C9 were 88 and 57%, respectively, in the injected beetles, and 39 and 22%, respectively, in the fed beetles. Deuterium labelling slightly increases the retention time of relevant ascarosides. (b) LC–MS/MS analysis of ascarosides inside beetles and on the surface of beetles grown at different temperatures.