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. 2016 Aug 2;7:12207. doi: 10.1038/ncomms12207

Figure 4. Complete deletion of the miR-17∼92 miRNA family impairs early B-cell development.

Figure 4

(a) B-cell percentages in the bone marrow of representative control and Mb1tKO mice are shown in the histograms and B-cell numbers in the bone marrow of all mice analysed are shown in the graph. (b) Representative plots of IgM+ B cells (B220+IgM+), pro-B cells (B220intckit+) and pre-B cells (B220intCD25+) in the bone marrow of control and Mb1tKO mice. (c) Cell numbers of all B-cell developmental stages in the bone marrow (Hardy fractions A to F) in control and Mb1tKO mice. (d) B-cell percentages in the spleen of representative control and Mb1tKO mice are shown in the histograms and splenic B-cell numbers are shown in the graph. (e) Apoptosis of pro-B and pre-B cells was measured by flow cytometry analysis of annexin V and active caspase 3. *P<0.05 and **P<0.01 (two-tailed Student's t-test). Data are representative of 2 (a,b,d) or pooled from 2 (c,d,e: active caspase 3) or 3 (e: Annexin V) independent experiments (mean±s.e.m. in c,d,e) with n=12 (Control and Mb1tKO) in a–d, 8 (Control and Mb1tKO) in e (Annexin V) and 5 (Control and Mb1tKO) in e (active caspase 3).