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. 2016 Aug 2;7:12217. doi: 10.1038/ncomms12217

Figure 2. Current production by anode biofilms of WT and mutants in MECs fed with 1 mM acetate.

Figure 2

(a) Representative plots of current generation by WT, pili-deficient strains (pilB, pilA and pilA-E5A) and omcZ mutant in MECs operated in batch and fed with an initial concentration of 1 mM acetate. Inset shows current generation (Y axis, in mA) over time (X axis, in days) by double mutants pilB mshE (solid grey), pilB gspE (dashed grey) and an equal mixture of pilB and omcZ cells (blue). (b,c) Confocal micrographs showing top and side views of anode biofilms of the WT (b) and pilB (c) strains collected at the end of the MEC experiment, when current had decreased to <0.1 mA, and stained with the BacLight viability kit (green, live; red, dead). Scale bar, 20 μm.