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. 2016 Aug 2;7:12328. doi: 10.1038/ncomms12328

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Copyright © 2016, The Author(s)

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(a) Deletion variants of HA-SALM4 (HA-S4). FL, full length. (b) Total and surface expression levels of SALM4 deletion variants. HEK293T cells expressing HA-SALM4 deletion variants were biotinylated, precipitated and immunoblotted. (c–e) Deletion variants of HA-SALM4 differently interact with SALM2/3/5 in heterologous cells. HEK293T cell lysates doubly transfected with HA-SALM4 (WT and deletion variants)+SALM2/3/5 (Myc-SALM2, SALM3-EGFP and SALM5-EGFP) were immunoprecipitated and immunoblotted with the indicated antibodies.