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. 2016 Aug 1;7:12348. doi: 10.1038/ncomms12348

Figure 1. BRAF inhibition induces paradoxical MAPK activation in keratinocytes (HEKa).

Figure 1

(a) Western blot analyses of pERK in HEKa compared with the BRAFV600E mutant melanoma cell line M249 when treated with vemurafenib. (b) Levels of pERK and pMEK in HEKa compared with the BRAFV600E mutant melanoma line M249 when treated with vemurafenib (VEM), trametinib (TRAME) or the combination for 24 h. (c) Histograms of intracellular flow cytometry analyses of HEKa and M249 cells treated with vehicle or vemurafenib (1.5 μM) and stained with pERK and Ki67 (staining controls represented in Supplementary Fig. 6). (d) Quantification of fold-change of pERK and Ki67 levels in three replicate cultures of HEKa and M249 cells treated with vemurafenib compared to vehicle. Error bars, mean±s.d.; n=3. Results are representative of two experiments.