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. 2016 Aug 1;7:12310. doi: 10.1038/ncomms12310

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Copyright © 2016, The Author(s)

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(a,b) hCINAP depletion caused cell cycle arrest at the G1 phase. HCT116 cells stably expressing control-shRNA or hCINAP-shRNA were subjected to FACS analysis. The percentage of cells in each phase was obtained from three independent experiments. (c,d) Apoptosis was measured with cells labelling with AnnexinV–fluorescein isothiocyanate (FITC) and propidium iodide and the signal was obtained using a BD FACS Caliber instrument. Results are presented as mean±s.d. **P<0.01 (Student's t-test). (e) HCT116 cells with stably expressed control-shRNA or hCINAP-shRNA were seeded in the soft agar and cultured for 2 weeks. Representative images of colonies formed by each type of cells were shown. Scale bar, 200 μm. (f) Tumour formation in nude mice (female, BALB/c, 5 weeks of age, 10 mice per group, randomly assigned) injected subcutaneously with HCT116 cells harbouring control-shRNA or hCINAP-shRNA. Tumours were isolated after 3 weeks. This experiment was carried out according to the ethical guidelines and approved by the Peking University Laboratory Animal Center.