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. 2016 Aug 4;31(8):1816–1826. doi: 10.1093/humrep/dew126

Figure 3.

Figure 3

Charged iron particles increase ovarian γH2AX immunostaining. Representative image of γH2AX immunostaining in granulosa cells (black arrow) and oocytes (arrowhead) of follicles (A), and negative control image with primary antibody replaced by nonimmune IgG (B). Graphs show the mean ± SEM percentage of follicles with γH2AX-positive granulosa cells or oocytes. (C) Six hours after irradiation, there were statistically significant differences in percentages of secondary and antral follicles with γH2AX-positive granulosa cells among groups (P < 0.05, Kruskal–Wallis tests). (D) At 6 h there were statistically significant differences in percentages of primordial, primary and secondary follicles with γH2AX-positive oocytes (P < 0.05, Kruskal–Wallis tests). (E) One week after irradiation, there were statistically significant differences in percentages of secondary and antral follicles with γH2AX-positive granulosa cells among groups (P < 0.05, Kruskal–Wallis tests). (F) At 1 week, there were no statistically significant differences in percentages of follicles with γH2AX-positive oocytes among groups. *P < 0.05 versus 0 cGy control by Mann–Whitney test; §P < 0.05, 50 cGY + ALA versus 50 cGy by Mann–Whitney test. n = 4 mice/group.