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. 2016 Aug 4;31(8):1816–1826. doi: 10.1093/humrep/dew126

Figure 5.

Figure 5

Charged iron particles increase protein nitration in ovarian follicles. (A) Representative image of NTY localization in granulosa cells (black arrow) and oocytes (arrowhead) of follicles. (B) Negative control image with primary antibody replaced by nonimmune IgG. Graphs show the mean ± SEM percentage of follicles with NTY-positive granulosa cells or oocytes; 6 h after irradiation, NTY immunostaining in granulosa cells did not vary among groups (P > 0.05, Kruskal–Wallis test) (C), while percentages of primordial and secondary follicles with NTY-positive oocytes varied significantly among groups (P < 0.05, Kruskall–Wallis test) (D). One week after irradiation, percentages of follicles with NTY-positive granulosa cells (E) and percentages of follicles with NTY-positive oocytes did not vary among groups (P > 0.05, Kruskal–Wallis test). *P < 0.05 versus 0 cGy control by Mann–Whitney test; §P < 0.05, 50 cGY + ALA versus 50 cGy by Mann–Whitney test. n = 4 mice/group.