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. 2016 Aug 4;31(8):1816–1826. doi: 10.1093/humrep/dew126

Figure 6.

Figure 6

Charged iron particles increase caspase-3 activation in ovarian follicles. (A) Representative image of activated caspase-3 localization in granulosa cells (black arrow) and oocytes (arrowhead) of follicles. (B) Negative control with primary antibody replaced by nonimmune IgG. Graphs show the means ± SEM percentages of follicles with caspase-3 positive granulosa cells or oocytes. (C) At 6 h after irradiation, the effect of treatment group on percentages of follicles with caspase 3-positive granulosa cells approached significance for primordial follicles (P = 0.07, Kruskal–Wallis test) and was statistically significant for secondary follicles (P < 0.05, Kruskal–Wallis test). (D) Caspase-3 immunostaining in oocytes did not vary among groups at 6 h. (E) One week after irradiation, the percentage of secondary follicles with activated caspase 3-positive granulosa cells varied significantly among groups (P < 0.05, Kruskal–Wallis test). (F) One week after irradiation, percentages of primordial and primary follicles with activated caspase 3 positive oocytes varied significantly among groups (P < 0.05, Kruskal–Wallis test). *P < 0.05 versus 0 cGy control by Mann–Whitney test; §P < 0.05, 50 cGY + ALA versus 50 cGy by Mann–Whitney test. n = 4 mice/group.