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. 2016 Aug 5;47:75. doi: 10.1186/s13567-016-0363-8

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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qRT-PCR validation and kinetic analysis of ISG expression. (A) Validation of RNA-seq and Microarray ISGs by qRT-PCR of CEF treated with recombinant chicken IFN1 (1000 units, 6 h). (B) Kinetics of expression of selected ISGs assayed by qRT-PCR following treatment of CEF with recombinant chicken IFN1 (1000 units) for 45, 90, 180 or 360 min. ISGs showing similar kinetic expression profiles are paired vertically.