Fig. 1.

SMARCE1 knockdown reduces lung metastasis of breast cancer in vivo. a Expression levels of SMARCE1 mRNA and protein in LM-EV and LM-SMARCE1-KD cells. b Effect of SMARCE1 knockdown on the growth of xenografts in the fourth inguinal mammary fat pads of female NSG mice. c Spontaneous lung metastasis from orthotopic sites. Metastatic foci of tumor cells expressing red fluorescent protein on the dorsal surface of the left lung lobe were imaged 38 days after tumor cell inoculation (upper panel). The presence of tumor cells in the lungs was visualized by hematoxylin and eosin (H&E) staining of formalin-fixed lung sections (10 μM) and quantified by ImageJ software (lower panel). d Number of circulating tumor cells in blood. Fluorescent tumor cells in mouse blood were isolated and counted 38 days after tumor cell inoculation. EV empty vector, KD knockdown, LM lung metastatic cell line derived from MDA-MB-231