Fig. 3.

SMARCE1 knockdown promotes cell migration, but sensitize cells to anoikis. a Effect of SMARCE1 knockdown in LM cells on cell migration and invasion. Boyden chambers, uncoated or coated with Matrigel were used to measure cell migratory and invasive potential, respectively. b Effect of SMARCE1 knockdown in LM cells on viability of cells cultured in dishes coated with polyHEMA to prevent adhesion. c Effect of SMARCE1 knockdown in HCC38 cells. d SMARCE1 overexpression in BT549 cells. e Blocking SMARCE1 expression by siRNA enhanced detachment-induced caspase activation in LM and HCC38 cells. Accell SMARTpool of SMARCE1-siRNA (1 μM) were delivered into cells 48 h prior to detachment. Caspase activities were determined at indicated time points after cell detachment by using the Caspase-Glo 3/7 assay kit. *p < 0.05, Student t test. EV empty vector, KD knockdown, LM lung metastatic cell line derived from MDA-MB-231