Fig. 6.

SMARCE1 collaborates with HIF1A to activate PTK2 transcription in detached cells. a Detachment-induced PTK2 mRNA expression is abolished by SMARCE1 knockdown in LM and HCC38 cells, whereas enhanced by SMARCE1 overexpression in BT549 cells. b Detachment-induced recruitment of HIF1A and SMARCA4 to the promoter region of PTK2 is diminished by SMARCE1 knockdown in LM cells. Chromatin binding of HIF1A, SMARCE1, and SMARCA4 was measured by ChIP-qPCR assay. Average fold enrichment of PTK2 promoter DNA by indicated antibodies (vs. control IgG) from three independent experiments were presented. c Detachment induces interaction between SMARCE1 and HIF1A in LM-EV and LM-SMARCE1-KD cells. The protein interaction was examined by immunoprecipitation/immunoblotting assays. *p < 0.05, Student t test. EV empty vector, KD knockdown, LM lung metastatic cell line derived from MDA-MB-231