Fig. 6.

High correlation between replicates suggests tight regulation of gene expression at each stage of Platynereis embryogenesis. a A Spearman correlation coefficient plot for one set of technical replicates. Each dot represents the expression value (FPKM) in two technical replicates at 6 h post fertilization. The Spearman correlation coefficient is 0.99 between the two technical replicates shown, and is 0.99 or higher for all other technical replicates. b A Spearman correlation coefficient plot for biological replicates at 6 h post fertilization. The Spearman correlation coefficient is 0.93 between the two biological replicates shown. The correlation coefficient ranges from 0.91 to 0.97 between all biological replicates. c A Spearman correlation coefficient heatmap for all seven stages sampled in our Platynereis transcriptome. Biological replicates are denoted with the purple brackets, and the additional technical replicates have blue brackets. The heatmap shows that each individual stage shows the highest correlation to the adjacent stages