Table 2.
Forward (F) and reverse (R) primer pairs used for polymerase chain reactions to detect hepatic specific gene transcripts in human adipose-derived stem cells, induced heaptocytes, and primary human hepatocytes
| Primer | Sequence (5′–3′) |
|---|---|
| β-actin | F: TGGACTTCGAGCAAGAGATG |
| R: GAAGGAAGGCTGGAAGAGTG | |
| CYP1A2 | F: CTTCGCTACCTGCCTAACCC |
| R: GACTGTGTCAAATCCTGCTCC | |
| CYP1A1 | F: CAAGGGGCGTTGTGTCTTTG |
| R: GTCGATAGCACCATCAGGGG | |
| CYP2A6 | F: CAGCACTTCCTGAATGAG |
| R: AGGTGACTGGGAGGACTTGAGGC | |
| CYP2B6 | F: GCACTCCTCACAGGACTCTTG |
| R: CCCAGGTGTACCGTGAAGAC | |
| CYP2C9 | F: CTACAGATAGGTATTAAGGACA |
| R: GCTTCATATCCATGCAGCACCAC | |
| CYP2D6 | F: TGAAGGATGAGGCCGTCTGGGAGA |
| R: CAGTGGGCACCGAGAAGCTGAAGT | |
| CYP3A4 | F: TTCAGCAAGAAGAACAAGGACAA |
| R: GGTTGAAGAAGTCCTCCTAAGC |