Table 2.
Specific primers used in the study to target different bacterial groups and species of Bifidobacterium based on the 16S and groEL gene.
| Target species | Primer | Target gene |
Sequence (5′ to 3′) | Amplicon size |
Reference |
|---|---|---|---|---|---|
| All bacteria | Uni_V4_F | 16S | CAGCAGCCGCGGTAATAC | 389 | (39) |
| Uni_V4_R | CCGTCAATTTCTTTGAGTTT | ||||
| All bacteria | Uni_F | 16S | GTGSTGCAYGGYYGTCGTCA | 194 | (41) |
| Uni_R | ACGTCRTCCMCNCCTTCCTC | ||||
| All bacteria | 8F | 16S | AGAGTTTGATCCTGGCTCAG | 330 | (30), (31), (32) |
| 8F_B | AGGGTTCGATTCTGGCTCAG | ||||
| Uni_338_R | GCTGCCTCCCGTAGGAGT | ||||
| Actinobacteria | Actino_235_F | 16S | GCGKCCTATCAGCTTGTT | 283 | (42) |
| Actino_567_R | CCGCCTACGAGCYCTTTACGC | ||||
| Bifidobacteriaceae | Bifidobac_F | 16S | CTCCTGGAAACGGGTGG | 442 | (39) |
| Bifidobac_R | CTTCACACCRGACGCG | ||||
| Bifidobacterium | Bifido_5 | 16S | GATTCTGGCTCAGGATGAACGC | 236 | (43) |
| Bifido_3 | CTGATAGGACGCGACCCCAT | ||||
| B. adolescentis | Biado_1a | 16S | CTCCAGTTGGATGCATGTC | 279 | (44) |
| Biado_1b | TCCAGTTGACCGCATGGT | ||||
| Biado_2 | CGAAGGCTTGCTCCCAGT | ||||
| B. longum | Longum_5 | 16S | TTCCAGTTGATCGCATGGTCTTCT | 277 | (43) |
| Longum_3 | GGCTACCCGTCGAAGCCACG | ||||
| B. catenulatum group | BiCATg_1 | 16S | CGGATGCTCCGACTCCT | 285 | (44) |
| BiCATg_2 | CGAAGGCTTGCTCCCGAT | ||||
| B. bifidum | Bifidum_F | 16S | TGA CCG ACC TGC CCC ATG CT | 110 | (45) |
| Bifidum_R | CCC ATC CCA CGC CGA TAG AAT | ||||
| B. breve | BiBRE_F | 16S | CCGGATGCTCCATCACAC | 288 | (44) |
| BiBRE_R | ACAAAGTGCCTTGCTCCCT | ||||
| B. animalis | ISR_lactis_F | ISR | ATCCGAACTGAGACCGGTT | 382 | (40) |
| ISR_lactis_R | GCATGTTGCCAGCGGGTGA | ||||
| B. angulatum | BiAng_1 | 16S | CAGTCCATCGCATGGTGGT | 280 | (44) |
| BiAng_2 | GAAGGCTTGCTCCCCAAC | ||||
| B. dentium | BiDEN_1 | 16S | ATCCCGGGGGTTCGCCT | 387 | (44) |
| BiDEN_2 | GAAGGGCTTGCTCCCGA | ||||
| B. gallicum | BiGAL_1 | 16S | TAATACCGGATGTTCCGCTC | 303 | (44) |
| BiGAL_2 | ACATCCCCGAAAGGACGC | ||||
| B. adolescentis | B_ado_F | groEL | CTCCGCCGCTGATCCGGAAGTCG | 268 | (46) |
| B_ado_R | AACCAACTCGGCGATGTGGACGACA | ||||
| B.longum | B_lon_F | groEL | CGGCGTYGTGACCGTTGAAGAC | 259 | (46) |
| B_lon_R | TGYTTCGCCRTCGACGTCCTCA | ||||
| Catenulatum group | B_cat_F | groEL | GGCTATCGTCAAGGAGCTCA | 188 | (46) |
| B_cat_R | AGTCCAGATCCAAACCGAAAC | ||||
| B. bifidum | B_bif_F | groEL | CTCCGCAGCCGACCCCGAGGTT | 233 | (46) |
| B_bif_R | TGGAAACCTTGCCGGAGGTCAGG | ||||
| B. breve | B_bre_F | groEL | GCTCGTCGTTGCCGCCAAGGACGTT | 272 | (46) |
| B_bre_R | ACAGAATGTACGGATCCTCGAGCACG | ||||
| B. animalis | B_ani_F | groEL | CACCAATGCGGAAGACCAG | 184 | (46) |
| B_ani_R | GTTGTTGAGAATCAGCGTGG | ||||
| B. angulatum | B_ang_F | groEL | CTGTCCTCCCAGCAGGACGTGGTC | 97 | (46) |
| B_ang_R | GCGCTTCGCCGTCAACGTCTTCGG | ||||
| B. dentium | B_den_F | groEL | GGCCCAGTCTTTGGTGCATGAAGGCC | 364 | (46) |
| B_den_R | GTCTTCGAGCACCGCGGTCTGGTCC | ||||
| B. gallicum | B_gal_F | groEL | AGCTCGTCAAGTCCGCCAAGC | 188 | (46) |
| B_gal_R | CATACCTTCGGTGAACTCGAGG |