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. 1982 Feb;35(2):144–150. doi: 10.1136/jcp.35.2.144

Immunohistological study of human lungs by immunoperoxidase technique.

B Fox, S Shousha, K R James, G C Miller
PMCID: PMC497487  PMID: 7040481

Abstract

An unlabelled antibody peroxidase-antiperoxidase method for the detection of IgG, IgM, complement (C3 and Clq), fibrinogen and albumin was applied to routinely processed paraffin sections of lung from 27 cases. The results in 11 cases were compared with those obtained by immunofluorescence using frozen sections. Tissue was obtained from surgical specimens of cases with interstitial pneumonia comprising 10 of the usual type (UIP) and three of the desquamative type (DIP). Tissue was also obtained from the specimens of cases with sarcoidosis (two cases) and granulomatous inflammation of unknown cause (one case). There were 11 control cases, nine with primary carcinoma of the lung and two with metastatic tumours of the lung. Immunoglobulins of various types and complement were seen in diseased lung tissue. Although most of these deposits were probably due to a non-immunological mechanism there was evidence of the possible implication of immune complexes in three cases of UIP and in the interstitial pneumonia present in the two cases of sarcoidosis. The immunoperoxidase technique is a more sensitive method than immunofluorescence and has the additional advantage of the easy identification of the precise sites of the various deposits.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ainslie W. H., Jr, Britt K., Moshipur J. A. Maternal death due to lupus pneumonitis in pregnancy. Mt Sinai J Med. 1979 Sep-Oct;46(5):494–499. [PubMed] [Google Scholar]
  2. Beirne G. J., Octaviano G. N., Kopp W. L., Burns R. O. Immunohistology of the lung in Goodpasture's syndrome. Ann Intern Med. 1968 Dec;69(6):1207–1212. doi: 10.7326/0003-4819-69-6-1207. [DOI] [PubMed] [Google Scholar]
  3. Border W. A., Baehler R. W., Bhathena D., Glassock R. J. IgA antibasement membrane nephritis with pulmonary hemorrhage. Ann Intern Med. 1979 Jul;91(1):21–25. doi: 10.7326/0003-4819-91-1-21. [DOI] [PubMed] [Google Scholar]
  4. Brentjens J., Ossi E., Albini B., Sepulveda M., Kano K., Sheffer J., Vasilion P., Marine E., Baliah T., Jockin H. Disseminated immune deposits in lupus erythematosus. Arthritis Rheum. 1977 May;20(4):962–968. doi: 10.1002/art.1780200408. [DOI] [PubMed] [Google Scholar]
  5. Capron J. P., Marti R., Rey J. L., Dupas J. L., Capron D., Delamarre J., Potet F. Fibrosing alveolitis and hepatitis B surface antigen-associated chronic active hepatitis in a patient with immunoglobulin A deficiency. Am J Med. 1979 May;66(5):874–878. doi: 10.1016/0002-9343(79)91141-0. [DOI] [PubMed] [Google Scholar]
  6. Daniele R. P., McMillan L. J., Dauber J. H., Rossman M. D. Immune complexes in sarcoidosis: a correlation with activity and duration of disease. Chest. 1978 Sep;74(3):261–264. doi: 10.1378/chest.74.3.261. [DOI] [PubMed] [Google Scholar]
  7. DeHoratius R. J., Abruzzo J. L., Williams R. C., Jr Immunofluorescent and immunologic studies of rheumatoid lung. Arch Intern Med. 1972 Mar;129(3):441–446. [PubMed] [Google Scholar]
  8. DeLellis R. A., Sternberger L. A., Mann R. B., Banks P. M., Nakane P. K. Immunoperoxidase technics in diagnostic pathology. Report of a workshop sponsored by the National Cancer Institute. Am J Clin Pathol. 1979 May;71(5):483–488. doi: 10.1093/ajcp/71.5.483. [DOI] [PubMed] [Google Scholar]
  9. Dreisin R. B., Schwarz M. I., Theofilopoulos A. N., Stanford R. E. Circulating immune complexes in the idiopathic interstitial pneumonias. N Engl J Med. 1978 Feb 16;298(7):353–357. doi: 10.1056/NEJM197802162980701. [DOI] [PubMed] [Google Scholar]
  10. Eagen J. W., Memoli V. A., Roberts J. L., Matthew G. R., Schwartz M. M., Lewis E. J. Pulmonary hemorrhage in systemic lupus erythematosus. Medicine (Baltimore) 1978 Nov;57(6):545–560. doi: 10.1097/00005792-197811000-00005. [DOI] [PubMed] [Google Scholar]
  11. Ghose T., Landrigan P., Asif A. Localization of immunoglobulin and complement in pulmonary sarcoid granulomas. Chest. 1974 Sep;66(3):264–268. doi: 10.1378/chest.66.3.264. [DOI] [PubMed] [Google Scholar]
  12. Ghose T., Landrigan P., Killeen R., Dill J. Immunopathological studies in patients with farmer's lung. Clin Allergy. 1974 Jun;4(2):119–129. doi: 10.1111/j.1365-2222.1974.tb01369.x. [DOI] [PubMed] [Google Scholar]
  13. Gupta R. C., Kueppers F., DeRemee R. A., Huston K. A., McDuffie F. C. Pulmonary and extrapulmonary sarcoidosis in relation to circulating immune complexes: a quantification of immune complexes by two radioimmunoassays. Am Rev Respir Dis. 1977 Aug;116(2):261–266. doi: 10.1164/arrd.1977.116.2.261. [DOI] [PubMed] [Google Scholar]
  14. Hedfors E., Norberg R. Evidence for circulating immune complexes in sarcoidosis. Clin Exp Immunol. 1974 Mar;16(3):493–496. [PMC free article] [PubMed] [Google Scholar]
  15. Heyderman E. Immunoperoxidase technique in histopathology: applications, methods, and controls. J Clin Pathol. 1979 Oct;32(10):971–978. doi: 10.1136/jcp.32.10.971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hogan P. G., Donald K. J., McEvoy J. D. Immunofluorescence studies of lung biopsy tissue. Am Rev Respir Dis. 1978 Sep;118(3):537–545. doi: 10.1164/arrd.1978.118.3.537. [DOI] [PubMed] [Google Scholar]
  17. Iseman M. D., Schwarz M. I., Stanford R. E. Interstitial pneumonia in angio-immunoblastic lymphadenopathy with dysproteinemia. A case report with special histopathologic studies. Ann Intern Med. 1976 Dec;85(6):752–755. doi: 10.7326/0003-4819-85-6-752. [DOI] [PubMed] [Google Scholar]
  18. Johnson J. P., Whitman W., Briggs W. A., Wilson C. B. Plasmapheresis and immunosuppressive agents in antibasement membrane antibody-induced Goodpasture's syndrome. Am J Med. 1978 Feb;64(2):354–359. doi: 10.1016/0002-9343(78)90065-7. [DOI] [PubMed] [Google Scholar]
  19. Katz R. M., Kniker W. T. Infantile hypersensitivity pneumonitis as a reaction to organic antigens. N Engl J Med. 1973 Feb 1;288(5):233–237. doi: 10.1056/NEJM197302012880504. [DOI] [PubMed] [Google Scholar]
  20. Koffler D., Sandson J., Carr R., Kunkel H. G. Immunologic studies concerning the pulmonary lesions in Goodpasture's syndrome. Am J Pathol. 1969 Feb;54(2):293–305. [PMC free article] [PubMed] [Google Scholar]
  21. Markowitz A. S., Battifora H. A., Schwartz F., Aseron C. Immunological aspects of Goodpasture's syndrome. Clin Exp Immunol. 1968 Jul;3(6):585–591. [PMC free article] [PubMed] [Google Scholar]
  22. Martinez J. S., Kohler P. F. Variant "Goodpasture's syndrome"? The need for immunologic criteria in rapidly progressive glomerulonephritis and hemorrhagic pneumonitis. Ann Intern Med. 1971 Jul;75(1):67–76. doi: 10.7326/0003-4819-75-1-67. [DOI] [PubMed] [Google Scholar]
  23. May J. J., Schwarz M. I., Dreisin R. B. Idiopathic thrombocytopenic purpura occurring with interstitial pneumonitis. Ann Intern Med. 1979 Feb;90(2):199–200. doi: 10.7326/0003-4819-90-2-199. [DOI] [PubMed] [Google Scholar]
  24. Pertschuk L. P., Moccia L. F., Rosen Y., Lyons H., Marino C. M., Rashford A. A., Wollschlager C. M. Acute pulmonary complications in systemic lupus erythematosus. Immunofluorescence and light microscopic study. Am J Clin Pathol. 1977 Nov;68(5):553–557. doi: 10.1093/ajcp/68.5.553. [DOI] [PubMed] [Google Scholar]
  25. Pratt D. S., Schwartz M. I., May J. J., Dreisin R. B. Rapidly fatal pulmonary fibrosis: the accelerated variant of interstitial pneumonitis. Thorax. 1979 Oct;34(5):587–593. doi: 10.1136/thx.34.5.587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Rodríguez-Iturbe B., García R., Rubio L., Serrano H. Immunohistologic findings in the lung in systemic lupus erythematosus. Arch Pathol Lab Med. 1977 Jul;101(7):342–344. [PubMed] [Google Scholar]
  27. Scadding J. G., Hinson K. F. Diffuse fibrosing alveolitis (diffuse interstitial fibrosis of the lungs). Correlation of histology at biopsy with prognosis. Thorax. 1967 Jul;22(4):291–304. doi: 10.1136/thx.22.4.291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Schatz M., Patterson R., Fink J. Immunologic lung disease. N Engl J Med. 1979 Jun 7;300(23):1310–1320. doi: 10.1056/NEJM197906073002305. [DOI] [PubMed] [Google Scholar]
  29. Schwarz M. I., Dreisin R. B., Pratt D. S., Stanford R. E. Immunofluorescent patterns in the idiopathic interstitial pneumonias. J Lab Clin Med. 1978 Jun;91(6):929–938. [PubMed] [Google Scholar]
  30. Teichman S., Briggs W. A., Knieser M. R., Enquist R. W. Goodpasture's syndrome: two cases with contrasting early course and management. Am Rev Respir Dis. 1976 Feb;113(2):223–232. doi: 10.1164/arrd.1976.113.2.223. [DOI] [PubMed] [Google Scholar]
  31. Turner-Warwick M. Philip Ellman lecture. Immunological aspects of systemic diseases of the lungs. Proc R Soc Med. 1974 Jun;67(6 Pt 2):541–547. [PMC free article] [PubMed] [Google Scholar]
  32. Yeo P. P., Sinniah R. Lupus cor pulmonale with electron microscope and immunofluroescent antibody studies. Ann Rheum Dis. 1975 Oct;34(5):457–460. doi: 10.1136/ard.34.5.457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. van Toorn D. W. Coffee worker's lung. A new example of extrinsic allergic alveolitis. Thorax. 1970 Jul;25(4):399–405. doi: 10.1136/thx.25.4.399. [DOI] [PMC free article] [PubMed] [Google Scholar]

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