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Expression of Wnt5a after injection of AdWnt5a. (a-b) The dorsal skin of C57 mice at postnatal day 56 was depilated, and AdGFP or AdWnt5a were injected into the depilated skin. Expression of Wnt5a was determined by immunohistochemistry 3 days after injection. (a) AdGFP-treated group. (b) AdWnt5a-treated group. (c) Cultured JB6 cells were treated with AdWnt5a or AdGFP. The expression of Wnt5a was determined by RT-PCR 36 hours after treatment. Scale bar= 25 μm.
