Figure 5.

Downregulation of p-p38α protein level may be induced by a direct interaction between NLS-RARα and p38α protein. (A) Immunofluorescence microscopy of NLS-RARα staining with FITC(green) and p38α protein staining with TRITC(red) in NLS-RARα-overexpressed NB4 cells; (B, C) NLS-RARα was immunoprecipitated by anti-RARα, p38α protein(Myc-tagged) was detected by Western blot; (D) p38α was immunoprecipitated by anti-Myc, NLS-RARα (HA-tagged) was detected by Western blot. IgG was used as control antibody. The overexpressions of p38α protein and NLS-RARα(input) were determineded with Western blot analysis.