Figure 6.

Recruitment p38α-combinded NLS-RARα by ATRA eventually caused activation of p38α protein. (A) Western blot analysis of the expressions of p38α, p-p38α, NLS-RARα, C/EBPβ and CD11b proteins in cultured NLS-RARα-overexpressed NB4 cells after dealing cells with various treatments for 3 days. (B) The proliferation of NLS-RARα-overexpressed NB4 cells in different groups was determined with CCK-8 Kit. (C) NLS-RARα(HA-tagged) was co-immunoprecipitated by anti-Myc in different groups. (D) The expressions of p38α protein(Myc-tagged) and p-p38α protein in different groups. All data are presented as mean±SD. * p < 0.05. (1: negative group(DMSO-treated); 2: experimental group treated with 10 nM ATRA; 3: experimental group treated with 10 nM ATRA+10 μM PD169316)